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bead size gel filtration column  (Bio-Rad)


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    Bio-Rad bead size gel filtration column
    Fig. 4. SDS polyacrylamide gel electrophoresis of purified and partially purified endo-β-1,4-glucanase and laminarinase from the midgut gland of C. destructor and G. natalis. Gels were silver stained. (A) Lane 1, molecular mass standards with their sizes indicated in kDa. Lane 2, endo- β-1,4-glucanase purified from the midgut gland of G. natalis. Lane 3, endo- β-1,4-glucanase 2 purified from the midgut gland of C. destructor. Lane 4, laminarinase purified from the midgut gland of G. natalis. Lanes 5 and 6, laminarinases partially purified from midgut gland of C. destructor using either Bio-Rad P-100 gel <t>filtration</t> chromatography (Lane 5) or Mono-Q strong anion chromatography (Lane 6) as the third purification step. (B) Lane 1, endo-β-1,4-glucanase 1 purified from the midgut gland of C. destructor. Lane 2, molecular mass standards with their masses indicated in kDa.
    Bead Size Gel Filtration Column, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 96/100, based on 3948 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/bead size gel filtration column/product/Bio-Rad
    Average 96 stars, based on 3948 article reviews
    bead size gel filtration column - by Bioz Stars, 2026-05
    96/100 stars

    Images

    1) Product Images from "Purification and characterisation of endo-beta-1,4-glucanase and laminarinase enzymes from the gecarcinid land crab Gecarcoidea natalis and the aquatic crayfish Cherax destructor."

    Article Title: Purification and characterisation of endo-beta-1,4-glucanase and laminarinase enzymes from the gecarcinid land crab Gecarcoidea natalis and the aquatic crayfish Cherax destructor.

    Journal: The Journal of experimental biology

    doi: 10.1242/jeb.017657

    Fig. 4. SDS polyacrylamide gel electrophoresis of purified and partially purified endo-β-1,4-glucanase and laminarinase from the midgut gland of C. destructor and G. natalis. Gels were silver stained. (A) Lane 1, molecular mass standards with their sizes indicated in kDa. Lane 2, endo- β-1,4-glucanase purified from the midgut gland of G. natalis. Lane 3, endo- β-1,4-glucanase 2 purified from the midgut gland of C. destructor. Lane 4, laminarinase purified from the midgut gland of G. natalis. Lanes 5 and 6, laminarinases partially purified from midgut gland of C. destructor using either Bio-Rad P-100 gel filtration chromatography (Lane 5) or Mono-Q strong anion chromatography (Lane 6) as the third purification step. (B) Lane 1, endo-β-1,4-glucanase 1 purified from the midgut gland of C. destructor. Lane 2, molecular mass standards with their masses indicated in kDa.
    Figure Legend Snippet: Fig. 4. SDS polyacrylamide gel electrophoresis of purified and partially purified endo-β-1,4-glucanase and laminarinase from the midgut gland of C. destructor and G. natalis. Gels were silver stained. (A) Lane 1, molecular mass standards with their sizes indicated in kDa. Lane 2, endo- β-1,4-glucanase purified from the midgut gland of G. natalis. Lane 3, endo- β-1,4-glucanase 2 purified from the midgut gland of C. destructor. Lane 4, laminarinase purified from the midgut gland of G. natalis. Lanes 5 and 6, laminarinases partially purified from midgut gland of C. destructor using either Bio-Rad P-100 gel filtration chromatography (Lane 5) or Mono-Q strong anion chromatography (Lane 6) as the third purification step. (B) Lane 1, endo-β-1,4-glucanase 1 purified from the midgut gland of C. destructor. Lane 2, molecular mass standards with their masses indicated in kDa.

    Techniques Used: Polyacrylamide Gel Electrophoresis, Purification, Staining, Filtration, Chromatography



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    Bio-Rad bead size gel filtration column
    Fig. 4. SDS polyacrylamide gel electrophoresis of purified and partially purified endo-β-1,4-glucanase and laminarinase from the midgut gland of C. destructor and G. natalis. Gels were silver stained. (A) Lane 1, molecular mass standards with their sizes indicated in kDa. Lane 2, endo- β-1,4-glucanase purified from the midgut gland of G. natalis. Lane 3, endo- β-1,4-glucanase 2 purified from the midgut gland of C. destructor. Lane 4, laminarinase purified from the midgut gland of G. natalis. Lanes 5 and 6, laminarinases partially purified from midgut gland of C. destructor using either Bio-Rad P-100 gel <t>filtration</t> chromatography (Lane 5) or Mono-Q strong anion chromatography (Lane 6) as the third purification step. (B) Lane 1, endo-β-1,4-glucanase 1 purified from the midgut gland of C. destructor. Lane 2, molecular mass standards with their masses indicated in kDa.
    Bead Size Gel Filtration Column, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/bead size gel filtration column/product/Bio-Rad
    Average 96 stars, based on 1 article reviews
    bead size gel filtration column - by Bioz Stars, 2026-05
    96/100 stars
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    Fig. 4. SDS polyacrylamide gel electrophoresis of purified and partially purified endo-β-1,4-glucanase and laminarinase from the midgut gland of C. destructor and G. natalis. Gels were silver stained. (A) Lane 1, molecular mass standards with their sizes indicated in kDa. Lane 2, endo- β-1,4-glucanase purified from the midgut gland of G. natalis. Lane 3, endo- β-1,4-glucanase 2 purified from the midgut gland of C. destructor. Lane 4, laminarinase purified from the midgut gland of G. natalis. Lanes 5 and 6, laminarinases partially purified from midgut gland of C. destructor using either Bio-Rad P-100 gel filtration chromatography (Lane 5) or Mono-Q strong anion chromatography (Lane 6) as the third purification step. (B) Lane 1, endo-β-1,4-glucanase 1 purified from the midgut gland of C. destructor. Lane 2, molecular mass standards with their masses indicated in kDa.

    Journal: The Journal of experimental biology

    Article Title: Purification and characterisation of endo-beta-1,4-glucanase and laminarinase enzymes from the gecarcinid land crab Gecarcoidea natalis and the aquatic crayfish Cherax destructor.

    doi: 10.1242/jeb.017657

    Figure Lengend Snippet: Fig. 4. SDS polyacrylamide gel electrophoresis of purified and partially purified endo-β-1,4-glucanase and laminarinase from the midgut gland of C. destructor and G. natalis. Gels were silver stained. (A) Lane 1, molecular mass standards with their sizes indicated in kDa. Lane 2, endo- β-1,4-glucanase purified from the midgut gland of G. natalis. Lane 3, endo- β-1,4-glucanase 2 purified from the midgut gland of C. destructor. Lane 4, laminarinase purified from the midgut gland of G. natalis. Lanes 5 and 6, laminarinases partially purified from midgut gland of C. destructor using either Bio-Rad P-100 gel filtration chromatography (Lane 5) or Mono-Q strong anion chromatography (Lane 6) as the third purification step. (B) Lane 1, endo-β-1,4-glucanase 1 purified from the midgut gland of C. destructor. Lane 2, molecular mass standards with their masses indicated in kDa.

    Article Snippet: Gel filtration chromatography Protein concentrates from either the anion exchange chromatography step or the HIC step were applied to a 1.5cm 50cm P-100 medium bead size gel filtration column (Bio-Rad).

    Techniques: Polyacrylamide Gel Electrophoresis, Purification, Staining, Filtration, Chromatography